In Vitro Study of Iron Doped Hydroxyapatite

Abstract

The effect of iron substitution on the bioactivity of hydroxyapatite (HAp) under the physiological conditions was investigated. Five samples of iron doped hydroxyapatite (FeHAp) with different iron concentrations (0, 0.05, 0.1, 0.2, and 0.3 mol %) were synthesized by wet chemical method. The formation of bone like apatite layer on the surface of the samples was detected using X-ray diffraction (XRD) and Fourier transforms infrared (FTIR). The changes of the pH of SBF (simulated body fluid medium) were measured at pre-determined time intervals using a pH meter. The dissolution of calcium, phosphorus and iron ions in SBF medium was determined by single beam scanning spectrophotometer. XRD and FTIR results exhibit the formation of carbonate apatite layer on the surface of the immersed samples, which increased with the increase of iron content. The solubility and dissolution tests revealed that iron doped HAp samples had a higher solubility and dissolution rat than pure sample, which indicate that iron increase the bioactivity of HAp in vitro.